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Article
Nuclear small subunit rRNA group I intron variation among Beauveria spp provide tools for strain identification and evidence of horizontal transfer
Current Genetics
  • Brad S. Coates, Iowa State University
  • Richard L. Hellmich, Iowa State University
  • Leslie C. Lewis, Iowa State University
Document Type
Article
Publication Date
1-1-2002
DOI
10.1007/s00294-002-0317-8
Abstract

An optional group I intron was characterized at a single insertion point in nuclear small subunit rRNA (nuSSU rRNA) genes of the imperfect entomopathogenic fungi, Beauveria bassiana and B. brongniartii. Insertion points were conserved among nuSSU rRNA genes from 35 Beauveria isolates. PCR-RFLP and DNA sequencing identified 12 group I intron variants and were applied to the identification of strains isolated from insect hosts. Alignment of 383– 404-nt subgroup IB3 group I introns indicated that four insertion/deletion (indel) mutations were the main basis of fragment length variation. Phylogeny reconstruction using parsimony and neighbor-joining methods suggested six lineages may be present among nuSSU rRNA group I intron sequences from Beauveria and related ascomycete fungi. Terminal node placement of Beauveria introns conflicted with previously published phylogenies constructed from gene sequences, suggesting horizontal transfer of group I introns. PCRRFLP among introns provided a means for the differentiation of Beauveria isolates.

Comments

This article is from Current Genetics; 41 (2002); 414-424; doi: 10.1007/s00294-002-0317-8

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Copyright Owner
Springer-Verlag
Language
en
Date Available
2014-08-25
File Format
application/pdf
Citation Information
Brad S. Coates, Richard L. Hellmich and Leslie C. Lewis. "Nuclear small subunit rRNA group I intron variation among Beauveria spp provide tools for strain identification and evidence of horizontal transfer" Current Genetics Vol. 41 Iss. 6 (2002) p. 414 - 424
Available at: http://works.bepress.com/richard_hellmich/51/