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Article
Two sex-chromosome-linked microsatellite loci show geographic variance among North American Ostrinia nubilalis
Journal of Insect Science
  • Brad S. Coates, Iowa State University
  • Richard L. Hellmich, Iowa State University
Document Type
Article
Publication Date
9-9-2003
DOI
10.1673/031.003.2901
Abstract

PCR-based O. nubilalis population and pedigree analysis indicated female specificity of a (GAAAAT)n microsatellite, and male specificity of a CAYCARCGTCACTAA repeat unit marker. These loci were respectively named Ostrinia nubilalis W-chromosome 1 (ONW1) and O. nubilalis Z-chromosome 1 (ONZ1). Intact repeats of three, four, or five GAAAAT units are present among ONW1 alleles, and biallelic variation exists at the ONZ1 locus. Screening of 493 male at ONZ1 and 448 heterogametic females at ONZ1 and ONW1 loci from eleven North American sample sites was used to construct genotypic data. Analysis of molecular variance (AMOVA) and F-statistics indicated no female haplotype or male ONZ1 allele frequency differentiation between voltinism ecotypes. Four subpopulations from northern latitudes, Minnesota and South Dakota, showed the absence of a single female haplotype, a significant deviation of ONZ1 data from Hardy-Weinberg expectation, and low-level geographic divergence from other subpopulations. Low ONZ1 and ONW1 allele diversity could be attributed either to large repeat unit sizes, low repeat number, reduced effective population (Ne) size of sex chromosomes, or the result of recent O. nubilalisintroduction and population expansion, but likely could not be due to inbreeding. These sequences have been deposited in GenBank AF442958, and AY102618 to AY102620.

Comments

This article is from Journal of Insect Science ;3 (2003); 1-6; doi: 10.1673/031.003.2901

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-08-26
File Format
application/pdf
Citation Information
Brad S. Coates and Richard L. Hellmich. "Two sex-chromosome-linked microsatellite loci show geographic variance among North American Ostrinia nubilalis" Journal of Insect Science Vol. 3 Iss. 29 (2003) p. 1 - 6
Available at: http://works.bepress.com/richard_hellmich/38/