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Unlinked genetic loci control the reduced transcription of aminopeptidase N 1 and 3 in the European corn borer and determine tolerance to Bacillus thuringiensis Cry1Ab toxin
Insect Biochemistry and Molecular Biology
  • Brad S. Coates, Iowa State University
  • Douglas V. Sumerford, United States Department of Agriculture
  • Blair D. Siegfried, University of Nebraska–Lincoln
  • Richard L. Hellmich, Iowa State University
  • Craig A. Abel, Iowa State University
Document Type
Article
Disciplines
Publication Date
12-1-2013
DOI
10.1016/j.ibmb.2013.09.003
Abstract

Transgenic expression of Bacillus thuringiensis (Bt) crystalline (Cry) toxins by crop plants result in reduced insect feeding damage, but sustainability is threatened by the development of resistance traits in target insect populations. We investigated Bt toxin resistance trait in a laboratory colony of the European corn borer, Ostrinia nubilalis, selected for increased survival when exposed to Cry1Ab and correlated survival on Cry1Ab toxin with a constitutive ∼146.2 ± 17.3-fold reduction in midgut aminopeptidase N1 (apn1) transcript levels. A 7.1 ± 1.9-fold reduction apn3 transcript level was also correlated with Cry1Ab resistance. Quantitative trait locus (QTL) mapping identified a single major genome region controlling Cry1Ab resistance on linkage group 24 (LG24), and a minor QTL on LG27. Both QTL were independent of apn1 and apn3 loci on LG02. Positional mapping identified genetic markers that may assist in the identification of causal gene(s) within QTL intervals. This study indicates that genetic factor(s) may act in trans to reduce both apn1 andapn3 expression in Cry1Ab resistant O. nubilalis larvae, and suggest that gene regulatory pathways can influence Bt resistance traits. These findings show that gene interactions (epistasis) may influence Bt resistance in target insect populations.

Comments

This article is from Insect Biochemistry and Molecular Biology 43 (2013): 1152–1160, doi:10.1016/j.ibmb.2013.09.003.

Rights
This work is open access under a CC BY-NC-SA 3.0 license.
Language
en
Date Available
2014-07-04
File Format
application/pdf
Citation Information
Brad S. Coates, Douglas V. Sumerford, Blair D. Siegfried, Richard L. Hellmich, et al.. "Unlinked genetic loci control the reduced transcription of aminopeptidase N 1 and 3 in the European corn borer and determine tolerance to Bacillus thuringiensis Cry1Ab toxin" Insect Biochemistry and Molecular Biology Vol. 43 Iss. 12 (2013) p. 1152 - 1160
Available at: http://works.bepress.com/richard_hellmich/37/