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A single major QTL controls expression of larval Cry1F resistance trait in Ostrinia nubilalis (Lepidoptera: Crambidae) and is independent of midgut receptor genes
Genetica
  • Brad S. Coates, Iowa State University
  • Douglas V. Sumerford, Iowa State University
  • Miriam D. Lopez, United States Department of Agriculture
  • Haichuan Wang, University of Nebraska-Lincoln
  • Lisa M. Fraser, Iowa State University
  • Jeremy A. Kroemer, United States Department of Agriculture
  • Terence Spencer, University of Nebraska-Lincoln
  • Kyung S. Kim, Seoul National University
  • Craig A. Abel, United States Department of Agriculture
  • Richard L. Hellmich, Iowa State University
  • Blair D. Siegfried, University of Nebraska-Lincoln
Document Type
Article
Publication Date
8-1-2011
DOI
10.1007/s10709-011-9590-0
Abstract
The European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae), is an introduced crop pest in North America that causes major damage to corn and reduces yield of food, feed, and biofuel materials. The Cry1F toxin from Bacillus thuringiensis (Bt) expressed in transgenic hybrid corn is highly toxic to O. nubilalis larvae and effective in minimizing feeding damage. A laboratory colony of O. nubilalis was selected for high levels of Cry1F resistance (>12,000-fold compared to susceptible larvae) and is capable of survival on transgenic hybrid corn. Genetic linkage maps with segregating AFLP markers show that the Cry1F resistance trait is controlled by a single quantitative trait locus (QTL) on linkage group 12. The map position of single nucleotide polymorphism (SNP) markers indicated that midgut Bt toxin-receptor genes, alkaline phosphatase, aminopeptidase N, and cadherin, are not linked with the Cry1F QTL. Evidence suggests that genes within this genome interval may give rise to a novel Bt toxin resistance trait for Lepidoptera that appears independent of known receptor-based mechanisms of resistance.
Comments

This article is from Genetica; 139 (2011); 961-972; doi: 10.1007/s10709-011-9590-0

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-09-02
File Format
application/pdf
Citation Information
Brad S. Coates, Douglas V. Sumerford, Miriam D. Lopez, Haichuan Wang, et al.. "A single major QTL controls expression of larval Cry1F resistance trait in Ostrinia nubilalis (Lepidoptera: Crambidae) and is independent of midgut receptor genes" Genetica Vol. 139 Iss. 8 (2011) p. 961 - 972
Available at: http://works.bepress.com/richard_hellmich/30/