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Genetic similarity among pheromone and voltinism races of Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae)
Insect Molecular Biology
  • P.C.R.G. Marcon, University of Nebraska-Lincoln
  • D. B. Taylor, University of Nebraska-Lincoln
  • C. E. Mason, University of Delaware
  • Richard L. Hellmich, Iowa State University
  • B. D. Siegfried, University of Nebraska-Lincoln
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The genetic variability of seven European corn borer populations, Ostrinia nubilalis, from North America and Europe was assessed by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis and DNA sequencing. The nuclear ribosomal internal transcribed spacer 1 (ITS-1) region (≈ 500 base pair [bp]) and four mitochondrial (mtDNA) regions (1550 bp total) were examined. The smartweed borer, Ostrinia obumbratalis, and south-Western corn borer, Diatraea grandiosella, were used for comparisons. Of 106 restriction sites identified (80 in mtDNA and 26 in ITS-1), none differentiated geographical populations, pheromone races, or voltine ecotypes of the European corn borer. The lack of variation in the ITS-1 of European corn borer was confirmed by DNA sequence analysis. The genetic similarity of European corn borer populations, despite their wide geographical range and physiological differences, may be explained by a relatively recent origin for the voltinism and pheromone races, gene flow among races, and/or expansion from genetic bottlenecks.

This article is from Insect Molecular Biology; 8 (1999);213-221; doi:10.1046/j.1365-2583.1999.820213.x .

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
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Blackwell Science Ltd
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P.C.R.G. Marcon, D. B. Taylor, C. E. Mason, Richard L. Hellmich, et al.. "Genetic similarity among pheromone and voltinism races of Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae)" Insect Molecular Biology Vol. 8 Iss. 2 (1999) p. 213 - 221
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