Skip to main content
Article
Polymorphic CA/GT and GA/CT microsatellite loci for Ostrinia nubilalis (Lepidoptera: Crambidae)
Molecular Ecology Notes
  • Brad S. Coates, Iowa State University
  • Richard L. Hellmich, Iowa State University
  • Leslie C. Lewis, Iowa State University
Document Type
Article
Publication Date
1-1-2005
DOI
10.1111/j.1471-8286.2004.00806.x
Abstract
Ten polymorphic dinucleotide (CA/GT and GA/CT) microsatellite loci suitable for population genetic screening were characterized from enriched partial Ostrinia nubilalis genomic libraries. Sequence from 126 enriched small insert genomic library clones identified 25 CA/GT and 58 GA/CT loci that were unique. Perfect repeats tended to be short (n = 10–12). Ten microsatellites, PCR amplified from a Crawfordsville Iowa population showed a mean of 10 alleles per locus (range six to 20), and six of 10 loci showed heterozygote deficiency. Amplification of eight loci was observed in the sister species O. furnicalis.
Comments

This article is from Molecular Ecology Notes; 5 (2005); 10-12; doi: 10.1111/j.1471-8286.2004.00806.x

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Brad S. Coates, Richard L. Hellmich and Leslie C. Lewis. "Polymorphic CA/GT and GA/CT microsatellite loci for Ostrinia nubilalis (Lepidoptera: Crambidae)" Molecular Ecology Notes Vol. 5 Iss. 1 (2005) p. 10 - 12
Available at: http://works.bepress.com/richard_hellmich/18/