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Sequence variation in trypsin- and chymotrypsin-like cDNAs from the midgut of Ostrinia nubilalis : methods for allelic differentiation of candidate Bacillus thuringiensis resistance genes
Insect Molecular Biology
  • Brad S. Coates, Iowa State University
  • Richard L. Hellmich, Iowa State University
  • Leslie C. Lewis, Iowa State University
Document Type
Article
Publication Date
1-1-2006
DOI
10.1111/j.1365-2583.2006.00598.x
Abstract
Midgut expressed alkaline serine proteases of Lepidoptera function in conversion of Bacillus thuringiensis (Bt) protoxin to active toxin, and reduced level of transcript T23 is associated with Ostrinia nubilalis resistance to Dipel® Bt formulations. Three groups of trypsin- (OnT25, OnT23, and OnT3) and two chymotrypsin-like (OnC1 and OnC2) cDNAs were isolated from O. nubilalis midgut tissue. Intraspecific groupings are based on cDNA similarity and peptide phylogeny. Derived serine proteases showed a catalytic triad (His, Asp, and Ser; except transcript OnT23a), three substrate specificity-determining residues, and three paired disulphide bonds. RT-PCR indicated all transcripts are expressed in the midgut. Mendelian-inherited genomic markers for loci OnT23, OnT3 and OnC1 will be useful for association of alleles with bioassayed Bt toxin resistance phenotypes.
Comments

This article is from Insect Molecular Biology; 15 (2006); 13-24; doi: 10.1111/j.1365-2583.2006.00598.x

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
Date Available
2014-08-27
File Format
application/pdf
Citation Information
Brad S. Coates, Richard L. Hellmich and Leslie C. Lewis. "Sequence variation in trypsin- and chymotrypsin-like cDNAs from the midgut of Ostrinia nubilalis : methods for allelic differentiation of candidate Bacillus thuringiensis resistance genes" Insect Molecular Biology Vol. 15 Iss. 1 (2006) p. 13 - 24
Available at: http://works.bepress.com/richard_hellmich/14/