Article
Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling
Analytical Chemistry
Document Type
Article
Publication Date
5-7-2013
Disciplines
Abstract
We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis.
Additional Comments
Air Force Office of Scientific Research grant #: FA 9550-10-1-0235; NIH RO1 grant #: CA 089305, 105102; National Cancer Institute's In-vivo Cellular and Molecular Imaging Center grant #: CA 114747; Integrative Cancer Biology Program grant #: CA 112973; NIH/NCI PO1 grant #: CA034233; Leukemia and Lymphoma Society Translational Research grant #: R6223-07
DOI
10.1021/ac400479j
Citation Information
Richard H. Perry, David I. Bellovin, Emelyn H. Shroff, Ali I. Ismail, et al.. "Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling" Analytical Chemistry Vol. 85 Iss. 9 (2013) p. 4529 - 4262 ISSN: 0003-2700 Available at: http://works.bepress.com/richard-perry/3/
©2013 American Chemical Society