This research aimed to develop and validate a mathematical model to predict thermal environment, main physiological responses, and productive performance of broiler chickens throughout acclimatized housing equipped with negative pressure tunnel ventilation systems associated with evaporative pad cooling, misting system, or both systems. Experimental results, used to validate the model showed that standard errors between simulated and measured values were smaller than or equal to 1.27, except for relative humidity, daily feed intake, and respiration rate, which were 5.43%, 1.77 g/day, and 2.15 breathes/min, respectively. It was concluded that the proposed mathematical model simulated satisfactorily all response variables modeled, allowing simulation of several production scenarios.