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A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories
Chemistry and Physics Faculty Proceedings, Presentations, Speeches, Lectures
  • Catherine Chiafair, Nova Southeastern University
  • Dimitri Giarikos, Nova Southeastern University
  • Reza Razeghifard, Nova Southeastern University
Event Name/Location
Division of Chemical Education ACS national meeting in Dallas, TX March 2014
Document Type
Publication Date

A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene by liver microsomes using Gas Chromatography-Mass Spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis and data interpretation of complex mixtures, and collaborative work.

Citation Information
Catherine Chiafair, Dimitri Giarikos and Reza Razeghifard. "A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories" (2014)
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