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Article
Evaluation of the Anticancer Activity of Bioactive Fraction G Extracted from Pavetta crassipes in Malignant Brain Tumor Cell Lines
American Journal of Phytomedicine and Clinical Therapeutics
  • Rachel M. Wilcox, Cedarville University
  • Eric Huseman, Cedarville University
  • Stacy Lin, Cedarville University
  • Belinda O Darkwah, Cedarville University
  • M. O. Emeje, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria
  • K. S. Gamaniel, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria
  • A. Orisadipe, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria
  • N. Enwerem, Howard University
  • B. A. Kefas, University of Virginia
  • Rebecca J. Gryka, Cedarville University
  • Denise Simpson, Cedarville University
  • Samson Amos, Cedarville University
Document Type
Article
Publication Date
8-14-2017
DOI
10.21767/2321-2748.100329
Abstract

Objective: Natural products have served as sources of lead compounds that are commonly used in the treatment of human diseases including cancer. Pavetta crassipes has been widely demonstrated to have ethnopharmacological potential in the management of malaria, gastrointestinal conditions, central nervous system behavioral disorders, hypertension, and cancer. The goal of our study was to evaluate the biological and molecular effects of Fraction G, obtained from the plant Pavetta crassipes, on glioblastoma invasive growth and survival.

Methodology: The antiproliferative effects of Fraction G, obtained from Pavetta crassipes, was evaluated using the trypan blue exclusion, (3-(4, 5-Dimethylthiazol- 2yl)-2, 5-Diphenyltetrazolium Bromide; MTT), and lactate dehydrogenase (LDH) assays. Flow cytometry and Western blotting analyses were carried out to examine the effects of Fraction G on cell cycle check-points and its effects on epidermal growth factor receptor-mediated signaling of AKT and MAPK pathways.

Results: In this paper, we report that the Fraction G obtained from the plant Pavetta crassipes induced a reduction in glioma cell viability and proliferation as well as induced an increase in apoptosis as evidenced by cleaved PARP, increased caspase 3/7 activity, and cell cycle arrest in the G0/G1 check point. Furthermore, we report that Fraction G inhibited the phosphorylation of AKT and MAPK following EGF treatment.

Conclusion: Taken together, our results demonstrate that Fraction G has potent inhibitory effects on pathways involved in glioblastoma proliferation and survival.

Keywords
  • Glioblastoma,
  • cancer,
  • Pavetta crassipes,
  • natural products,
  • ethnopharmacology,
  • cell proliferation,
  • apoptosis
Citation Information
Rachel M. Wilcox, Eric Huseman, Stacy Lin, Belinda O Darkwah, et al.. "Evaluation of the Anticancer Activity of Bioactive Fraction G Extracted from Pavetta crassipes in Malignant Brain Tumor Cell Lines" American Journal of Phytomedicine and Clinical Therapeutics Vol. 5 Iss. 2 (2017) p. 1 - 10
Available at: http://works.bepress.com/rebecca_gryka/73/