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The Antiproliferative and Apoptotic Effects of Apigenin on Glioblastoma Cells
The Journal of Pharmacy and Pharmacology
  • Trevor Stump, Cedarville University
  • Brittany Santee, Cedarville University
  • Lauren P. Williams, Cedarville University
  • Rachel Kunze, Cedarville University
  • Chelsae Heinze, Cedarville University
  • Eric Huseman, Cedarville University
  • Rebecca J. Gryka, Cedarville University
  • Denise Simpson, Cedarville University
  • Samson Amos, Cedarville University
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OBJECTIVES: Glioblastoma (GBM) is highly proliferative, infiltrative, malignant and the most deadly form of brain tumour. The epidermal growth factor receptor (EGFR) is overexpressed, amplified and mutated in GBM and has been shown to play key and important roles in the proliferation, growth and survival of this tumour. The goal of our study was to investigate the antiproliferative, apoptotic and molecular effects of apigenin in GBM.

METHODS: Proliferation and viability tests were carried out using the trypan blue exclusion, MTT and lactate dehydrogenase (LDH) assays. Flow cytometry was used to examine the effects of apigenin on the cell cycle check-points. In addition, we determined the effects of apigenin on EGFR-mediated signalling pathways by Western blot analyses.

KEY FINDINGS: Our results showed that apigenin reduced cell viability and proliferation in a dose- and time-dependent manner while increasing cytotoxicity in GBM cells. Treatment with apigenin-induced is poly ADP-ribose polymerase (PARP) cleavage and caused cell cycle arrest at the G2M checkpoint. Furthermore, our data revealed that apigenin inhibited EGFR-mediated phosphorylation of mitogen-activated protein kinase (MAPK), AKT and mammalian target of rapamycin (mTOR) signalling pathways and attenuated the expression of Bcl-xL.

CONCLUSION: Our results demonstrated that apigenin has potent inhibitory effects on pathways involved in GBM proliferation and survival and could potentially be used as a therapeutic agent for GBM.

  • Apigenin,
  • apoptosis,
  • epidermal growth factor receptor,
  • glioblastoma,
  • proliferation
Citation Information
Trevor Stump, Brittany Santee, Lauren P. Williams, Rachel Kunze, et al.. "The Antiproliferative and Apoptotic Effects of Apigenin on Glioblastoma Cells" The Journal of Pharmacy and Pharmacology Vol. 69 Iss. 7 (2017) p. 907 - 916 ISSN: 2042-7158
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