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Article
Surface and ultrastructural characterization of raw and pretreated switchgrass
Bioresource Technology (2011)
  • Bryon S. Donohoe, National Renewable Energy Laboratory
  • Todd B. Vinzant, National Renewable Energy Laboratory
  • Richard T. Elander, National Renewable Energy Laboratory
  • Venkata Ramesh Pallapolu, Auburn University
  • Y. Y. Lee, Auburn University
  • Rebecca G. Ong, Michigan State University
  • Venkatesh Balan, Michigan State University
  • Bruce E. Dale, Michigan State University
  • Youngmi Kim, Purdue University
  • Nathan S. Mosier, Purdue University
  • Michael R. Ladisch, Purdue University
  • Matthew Falls, Texas A&M University
  • Mark T. Holtzapple, Texas A&M University
  • Rocio Sierra-Ramirez, Universidad de Los Andes - Colombia
  • Jian Shi, University of California, Riverside
  • Mirvat A. Ebrik, University of California, Riverside
  • Tim Redmond, University of California at Riverside
  • Bin Yang, University of California, Riverside
  • Charles E. Wyman, University of California at Riverside
  • Bonnie Hames, Ceres, Inc.
  • Steve Thomas, Ceres, Inc.
  • Ryan E. Warner, Genencor
Abstract
The US Department of Energy-funded Biomass Refining CAFI (Consortium for Applied Fundamentals and Innovation) project has developed leading pretreatment technologies for application to switchgrass and has evaluated their effectiveness in recovering sugars from the coupled operations of pretreatment and enzymatic hydrolysis. Key chemical and physical characteristics have been determined for pretreated switchgrass samples. Several analytical microscopy approaches utilizing instruments in the Biomass Surface Characterization Laboratory (BSCL) at the National Renewable Energy Laboratory (NREL) have been applied to untreated and CAFI-pretreated switchgrass samples. The results of this work have shown that each of the CAFI pretreatment approaches on switchgrass result in different structural impacts at the plant tissue, cellular, and cell wall levels. Some of these structural changes can be related to changes in chemical composition upon pretreatment. There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields.
Keywords
  • Pretreatment,
  • enzymatic hydrolysis,
  • biomass,
  • switchgrass,
  • microscopy
Publication Date
April 2, 2011
DOI
10.1016/j.biortech.2011.03.092
Publisher Statement
© 2011 Published by Elsevier Ltd.
Citation Information
Bryon S. Donohoe, Todd B. Vinzant, Richard T. Elander, Venkata Ramesh Pallapolu, et al.. "Surface and ultrastructural characterization of raw and pretreated switchgrass" Bioresource Technology Vol. 102 Iss. 24 (2011) p. 11097 - 11104 ISSN: 0960-8524
Available at: http://works.bepress.com/rebecca-ong/23/