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Application of cellulase and hemicellulase to pure xylan, pure cellulose, and switchgrass solids from leading pretreatments
Bioresource Technology (2011)
  • Jian Shi, University of California, Riverside
  • Mirvat A. Ebrik, University of California, Riverside
  • Bin Yang, University of California, Riverside
  • Rebecca G. Ong, Michigan State University
  • Venkatesh Balan, Michigan State University
  • Bruce E. Dale, Michigan State University
  • Venkata Ramesh Pallapolu, Auburn University
  • Y. Y. Lee, Auburn University
  • Youngmi Kim, Purdue University
  • Nathan S. Mosier, Purdue University
  • Michael R. Ladisch, Purdue University
  • Mark T. Holtzapple, Texas A&M University
  • Matthew Falls, Texas A&M University
  • Rocio Sierra-Ramirez, Universidad de Los Andes - Colombia
  • Bryon S. Donohoe, National Renewable Energy Laboratory
  • Todd B. Vinzant, National Renewable Energy Laboratory
  • Richard T. Elander, National Renewable Energy Laboratory
  • Bonnie Hames, Ceres, Inc.
  • Steve Thomas, Ceres, Inc.
  • Ryan E. Warner, Genencor
  • Charles E. Wyman, University of California at Riverside
Accellerase 1000 cellulase, Spezyme CP cellulase, β-glucosidase, Multifect xylanase, and beta-xylosidase were evaluated for hydrolysis of pure cellulose, pure xylan, and switchgrass solids from leading pretreatments of dilute sulfuric acid, sulfur dioxide, liquid hot water, lime, soaking in aqueous ammonia, and ammonia fiber expansion. Distinctive sugar release patterns were observed from Avicel, phosphoric acid swollen cellulose (PASC), xylan, and pretreated switchgrass solids, with accumulation of significant amounts of xylooligomers during xylan hydrolysis. The strong inhibition of cellulose hydrolysis by xylooligomers could be partially attributed to the negative impact of xylooligomers on cellulase adsorption. The digestibility of pretreated switchgrass varied with pretreatment but could not be consistently correlated to xylan, lignin, or acetyl removal. Initial hydrolysis rates did correlate well with cellulase adsorption capacities for all pretreatments except lime, but more investigation is needed to relate this behavior to physical and compositional properties of pretreated switchgrass.
  • pretreatment,
  • enzymatic hydrolysis,
  • biofuels,
  • adsorption,
  • xylooligomers
Publication Date
April 8, 2011
Publisher Statement
© 2011 Elsevier Ltd.
Citation Information
Jian Shi, Mirvat A. Ebrik, Bin Yang, Rebecca G. Ong, et al.. "Application of cellulase and hemicellulase to pure xylan, pure cellulose, and switchgrass solids from leading pretreatments" Bioresource Technology Vol. 102 Iss. 24 (2011) p. 11080 - 11088 ISSN: 0960-8524
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