Application of cellulase and hemicellulase to pure xylan, pure cellulose, and switchgrass solids from leading pretreatmentsBioresource Technology (2011)
Accellerase 1000 cellulase, Spezyme CP cellulase, β-glucosidase, Multifect xylanase, and beta-xylosidase were evaluated for hydrolysis of pure cellulose, pure xylan, and switchgrass solids from leading pretreatments of dilute sulfuric acid, sulfur dioxide, liquid hot water, lime, soaking in aqueous ammonia, and ammonia fiber expansion. Distinctive sugar release patterns were observed from Avicel, phosphoric acid swollen cellulose (PASC), xylan, and pretreated switchgrass solids, with accumulation of significant amounts of xylooligomers during xylan hydrolysis. The strong inhibition of cellulose hydrolysis by xylooligomers could be partially attributed to the negative impact of xylooligomers on cellulase adsorption. The digestibility of pretreated switchgrass varied with pretreatment but could not be consistently correlated to xylan, lignin, or acetyl removal. Initial hydrolysis rates did correlate well with cellulase adsorption capacities for all pretreatments except lime, but more investigation is needed to relate this behavior to physical and compositional properties of pretreated switchgrass.
- enzymatic hydrolysis,
Publication DateApril 8, 2011
Citation InformationJian Shi, Mirvat A. Ebrik, Bin Yang, Rebecca G. Ong, et al.. "Application of cellulase and hemicellulase to pure xylan, pure cellulose, and switchgrass solids from leading pretreatments" Bioresource Technology Vol. 102 Iss. 24 (2011) p. 11080 - 11088 ISSN: 0960-8524
Available at: http://works.bepress.com/rebecca-ong/21/