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Investigation of enzyme formulation on pretreated switchgrass
Bioresource Technology (2011)
  • Matthew Falls, West Texas A&M University
  • Jian Shi, University of California, Riverside
  • Mirvat A. Ebrik, University of California, Riverside
  • Tim Redmond, University of California at Riverside
  • Bin Yang, University of California, Riverside
  • Charles E. Wyman, University of California at Riverside
  • Rebecca G. Ong, Michigan State University
  • Venkatesh Balan, Michigan State University
  • Bruce E. Dale, Michigan State University
  • Venkata Ramesh Pallapolu, Auburn University
  • Y. Y. Lee, Auburn University
  • Youngmi Kim, Purdue University
  • Nathan S. Mosier, Purdue University
  • Michael R. Ladisch, Purdue University
  • Bonnie Hames, Ceres, Inc.
  • Steve Thomas, Ceres, Inc.
  • Bryon S. Donohoe, National Renewable Energy Laboratory
  • Todd B. Vinzant, National Renewable Energy Laboratory
  • Richard T. Elander, National Renewable Energy Laboratory
  • Ryan E. Warner, Genencor
  • Rocio Sierra-Ramirez, Universidad de Los Andes - Colombia
  • Mark T. Holtzapple, Texas A&M University
This work studied the benefits of adding different enzyme cocktails (cellulase, xylanase, β-glucosidase) to pretreated switchgrass. Pretreatment methods included ammonia fiber expansion (AFEX), dilute-acid (DA), liquid hot water (LHW), lime, lime + ball-milling, soaking in aqueous ammonia (SAA), and sulfur dioxide (SO2). The compositions of the pretreated materials were analyzed and showed a strong correlation between initial xylan composition and the benefits of xylanase addition. Adding xylanase dramatically improved xylan yields for SAA (+8.4%) and AFEX (+6.3%), and showed negligible improvement (0–2%) for the pretreatments with low xylan content (dilute-acid, SO2). Xylanase addition also improved overall yields with lime + ball-milling and SO2 achieving the highest overall yields from pretreated biomass (98.3% and 93.2%, respectively). Lime + ball-milling obtained an enzymatic yield of 92.3 kg of sugar digested/kg of protein loaded.
  • switchgrass,
  • pretreatment,
  • xylanase,
  • b-glucosidase,
  • enzymatic digestion
Publication Date
March 21, 2011
Publisher Statement
© 2011 Elsevier Ltd.
Citation Information
Matthew Falls, Jian Shi, Mirvat A. Ebrik, Tim Redmond, et al.. "Investigation of enzyme formulation on pretreated switchgrass" Bioresource Technology Vol. 102 Iss. 24 (2011) p. 11072 - 11079 ISSN: 0960-8524
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