Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologiesBioresource Technology (2011)
The objective of this work is to investigate the effects of cellulase loading and b-glucosidase supplementation on enzymatic hydrolysis of pretreated Dacotah switchgrass. To assess the difference among various pretreatment methods, the profiles of sugars and intermediates were determined for differently treated substrates. For all pretreatments, 72 h glucan/xylan digestibilities increased sharply with enzyme loading up to 25 mg protein/g-glucan, after which the response varied depending on the pretreatment method.For a fixed level of enzyme loading, dilute sulfuric acid (DA), SO2, and Lime pretreatments exhibited higher digestibility than the soaking in aqueous ammonia (SAA) and ammonia fiber expansion (AFEX).Supplementation of Novozyme-188 to Spezyme-CP improved the 72 h glucan digestibility only for theSAA treated samples. The effect of b-glucosidase supplementation was discernible only at the early phaseof hydrolysis where accumulation of cellobiose and oligomers is significant. Addition of b-glucosidase increased the xylan digestibility of alkaline treated samples due to the b-xylosidase activity present in Novozyme-188.
Publication DateMarch 31, 2011
Citation InformationVenkata Ramesh Pallapolu, Y. Y. Lee, Rebecca G. Ong, Venkatesh Balan, et al.. "Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies" Bioresource Technology Vol. 102 Iss. 24 (2011) p. 11115 - 11120 ISSN: 0960-8524
Available at: http://works.bepress.com/rebecca-ong/12/