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Selected Works of Rebecca Ong
Article
Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies
Bioresource Technology (2011)
  • Venkata Ramesh Pallapolu, Auburn University
  • Y. Y. Lee, Auburn University
  • Rebecca G. Ong, Michigan State University
  • Venkatesh Balan, Michigan State University
  • Bruce E. Dale, Michigan State University
  • Youngmi Kim, Purdue University
  • Nathan M. Mousier, Purdue University
  • Michael R. Ladisch, Purdue University
  • Matthew Falls, Texas A&M University
  • Mark T. Hotlzapple, Texas A&M University
  • Rocio Sierra-Ramirez, Universidad de Los Andes - Colombia
  • Jian Shi, University of California at Riverside
  • Mirvat A. Ebrik, University of California at Riverside
  • Ted Redmond, University of California at Riverside
  • Bin Yang, University of California at Riverside
  • Charles E. Wyman, University of California at Riverside
  • Bryon S. Donohoe, National Renewable Energy Laboratory
  • Todd B. Vinzant, National Renewable Energy Laboratory
  • Richard T. Elander, National Renewable Energy Laboratory
  • Bonnie Hames, Ceres, Inc.
  • Steve Thomas, Ceres, Inc.
  • Ryan E. Warner, Genencor
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Abstract
The objective of this work is to investigate the effects of cellulase loading and b-glucosidase supplementation on enzymatic hydrolysis of pretreated Dacotah switchgrass. To assess the difference among various pretreatment methods, the profiles of sugars and intermediates were determined for differently treated substrates. For all pretreatments, 72 h glucan/xylan digestibilities increased sharply with enzyme loading up to 25 mg protein/g-glucan, after which the response varied depending on the pretreatment method.For a fixed level of enzyme loading, dilute sulfuric acid (DA), SO2, and Lime pretreatments exhibited higher digestibility than the soaking in aqueous ammonia (SAA) and ammonia fiber expansion (AFEX).Supplementation of Novozyme-188 to Spezyme-CP improved the 72 h glucan digestibility only for theSAA treated samples. The effect of b-glucosidase supplementation was discernible only at the early phaseof hydrolysis where accumulation of cellobiose and oligomers is significant. Addition of b-glucosidase increased the xylan digestibility of alkaline treated samples due to the b-xylosidase activity present in Novozyme-188.
Keywords
  • pretreatment,
  • switchgrass,
  • cellulase,
  • b-glucosidase,
  • digestibility
Disciplines
Publication Date
March 31, 2011
DOI
10.1016/j.biortech.2011.03.085
Publisher Statement
© 2011 Elsevier Ltd. All rights reserved
Citation Information
Venkata Ramesh Pallapolu, Y. Y. Lee, Rebecca G. Ong, Venkatesh Balan, et al.. "Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies" Bioresource Technology Vol. 102 Iss. 24 (2011) p. 11115 - 11120 ISSN: 0960-8524
Available at: http://works.bepress.com/rebecca-ong/12/