Handout
Sequence Annotation & Designing Gene-specific qPCR Primers (computational)
Bio 480 Advanced Molecular Biology
(2016)
Description
This class tested protocol will guide students through the steps for the following activities:
- Obtaining and annotating genomic DNA and mRNA sequence information
- Designing primers for quantitative PCR (qPCR) analysis of a cDNA library
Keywords
- qPCR,
- sequence annotation,
- RNA-seq,
- active learning,
- gene expression analysis protocol
Disciplines
Publication Date
Fall October 31, 2016
Citation Information
This work is licensed under a Creative Commons Attribution-ShareAlike 3.0 United States License. Recommended citation: Enke, R. (2016) Sequence Annotation & Designing Gene-specific qPCR Primers. CSHL DNALC RNA-Seq for the Next Generation Working Group. http://www.rnaseqforthenextgeneration.org/profiles/raymond-enke.html#teaching
Creative Commons license
This work is licensed under a Creative Commons CC_BY-SA International License.