DNA barcoding is a methodology used for identification and classification of organisms using a highly variable portion of the genome universal to all species within a broad taxonomic group.  This short DNA sequence barcode is compared to a comprehensive sequence database and used in tandem with morphology to characterize or identify the organism of interest.  Barcoding applications include the detection of fraudulently labeled food, identification of new or previously uncharacterized species, and cataloging biodiversity within a geographical region. Here we demonstrate an array of geospatial tools integrated into the DNA Barcoding workflow including GPS, we-based geospatial data hosts, geospatial database development and Google Earth to create interactive maps containing relevant metadata for each sample collected. These methods can be applied in tandem with traditional DNA Barcoding analysis as a part of the new JMU Biology first year curriculum beginning in the Fall 16 semester.  Integration of these tools into BIO140 & 150 Foundations Lab activities will provide first year JMU Biology majors with a broad range of experiences to help clarify their interests as well as a hone a diverse skill set to address questions related to ecology, molecular biology and bioinformatics.