Epigenetic regulation of CRX binding to regulatory elements in the vertebrate retinaJames Madison University College of Science and Math Summer Symposium (2015)
The vertebrate retina is a light-sensitive, stratified layer of neuronal cells that lines the interior of the eye. Retinal neurons are responsible for converting light into electrochemical signals that ultimately allow the brain to process visual images. This specialized cellular function requires finely tuned regulation of gene expression during retinal development. CRX is a retina-specific transcription factor required for mature photoreceptor development in the retina. An epigenetic modification known as DNA methylation may play an important role in regulation of CRX dependent retinal genes. Using a previously characterized CRX ChIP-seq data set and bisulfite pyrosequencing of genomic DNA, we demonstrate that high levels of DNA methylation in or adjacent to consensus CRX binding sites are associated with a lack of CRX binding and transcriptional silencing in the mouse retina. Interestingly, CRX binding to demethylated regulatory regions is sometimes, but not always, associated with active transcription. Collectively these findings suggest an important role for epigenetics in transcriptional regulation of retina specific genes. Current studies are focused on characterizing epigenetic regulation of human homologs of murine retina-specific genes. Future studies will focus on biochemical interactions between CRX protein and methylated DNA.
Citation InformationAnnamarie Meinsen^*, Nicholas Dunham*, Mogan Hedden*, Courtney Stout*, et al.. "Epigenetic regulation of CRX binding to regulatory elements in the vertebrate retina" James Madison University College of Science and Math Summer Symposium (2015)
Available at: http://works.bepress.com/raymond_enke/37/