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Differential Role of Lipocalin 2 During Immune Complex-Mediated Acute and Chronic Inflammation in Mice
Arthritis and Rheumatology (formerly Arthritis and Rheumatism)
  • Rangaiah Shashidharamurthy, Philadelphia College of Osteopathic Medicine
  • Deepa Machiah
  • Jesse D. Aitken
  • Kalyani Putty
  • Gayathri Srinivasan
  • Benoit Chassaing
  • Charles A. Parkos
  • Periasamy Selvaraj
  • Matam Vijay-Kumar
Document Type
Article
Publication Date
4-1-2013
Abstract
OBJECTIVE: Lipocalin 2 (LCN-2) is an innate immune protein that is expressed by a variety of cells and is highly up-regulated during several pathologic conditions, including immune complex (IC)-mediated inflammatory/autoimmune disorders. However, the function of LCN-2 during IC-mediated inflammation is largely unknown. Therefore, this study was undertaken to investigate the role of LCN-2 in IC-mediated diseases. METHODS: The up-regulation of LCN-2 was determined by enzyme-linked immunosorbent assay in 3 different mouse models of IC-mediated autoimmune disease: systemic lupus erythematosus, collagen-induced arthritis, and serum-transfer arthritis. The in vivo role of LCN-2 during IC-mediated inflammation was investigated using LCN-2-knockout mice and their wild-type littermates. RESULTS: LCN-2 levels were significantly elevated in all 3 of the autoimmune disease models. Further, in an acute skin inflammation model, LCN-2-knockout mice exhibited a 50% reduction in inflammation, with histopathologic analysis revealing notably reduced immune cell infiltration as compared to wild-type mice. Administration of recombinant LCN-2 to LCN-2-knockout mice restored inflammation to levels observed in wild-type mice. Neutralization of LCN-2 using a monoclonal antibody significantly reduced inflammation in wild-type mice. In contrast, LCN-2-knockout mice developed more severe serum-induced arthritis compared to wild-type mice. Histologic analysis revealed extensive tissue and bone destruction, with significantly reduced neutrophil infiltration but considerably more macrophage migration, in LCN-2-knockout mice compared to wild-type mice. CONCLUSION: These results demonstrate that LCN-2 may regulate immune cell recruitment to the site of inflammation, a process essential for the controlled initiation, perpetuation, and resolution of inflammatory processes. Thus, LCN-2 may present a promising target in the treatment of IC-mediated inflammatory/autoimmune diseases.
PubMed ID
23280250
Comments

This article was published in Arthritis & Rheumatism, Volume 65, Issue 4, April 2013, Pages 1064-73.

The published version is available at http://dx.doi.org/10.1002/art.37840

Copyright © 2013 by the American College of Rheumatology

Citation Information
Rangaiah Shashidharamurthy, Deepa Machiah, Jesse D. Aitken, Kalyani Putty, et al.. "Differential Role of Lipocalin 2 During Immune Complex-Mediated Acute and Chronic Inflammation in Mice" Arthritis and Rheumatology (formerly Arthritis and Rheumatism) Vol. 65 Iss. 4 (2013) p. 1064 - 1073
Available at: http://works.bepress.com/rangaiah_shashidharamurthy/4/