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In vitro clonal propagation of bael (Aegle marmelos Corr.) CV. CISHB1 through enhanced axillary branching
Physiol. Mol. Biol. Plants (2008)
  • Rajesh Pati
Abstract
Rapid clonal micropropagation protocol of Aegle marmelos (L.) Corr. cv. CISH-B1 was achieved by nodal stem segment of mature bearing tree. Three centimeter long shoots having one axillary bud excised from 10-15th nodal region of shoots during September gave quick in vitro bud burst (5.33 days) when cultured on MS medium supplemented with BAP, 8.84 μM + IAA 5.7 μM. The maximum number of proliferated shoots (9.0/explant) were obtained on same medium supplemented with BAP 8.84 μM + IAA 5.7 μM. The micro shoots were rooted (100 %) on ½ strength MS medium supplemented with IBA 49.0 + IAA 5.7 μM. In vitro rooted plants were acclimatized on autoclaved coconut husk containing ½ strength MS plant salt mixture and under shade net house (50 % shade 70-80 % RH). The plants were established in the field after acclimatization. The micropropagated plants were tested for its genetic fidelity using 13 RAPD, 3 ISSR and 2 DAMD primers. Profile obtained by all the three Single Primer Amplification Reaction (SPAR) technique from mother tree and micropropagated plants revealed genetic integrity of micropropagated plants with that of mother tree.
Keywords
  • Micropropagation,
  • Aegle marmelos,
  • PCR,
  • ISSR,
  • RAPD,
  • DAMD,
  • SPAR
Publication Date
Winter October 14, 2008
Citation Information
Rajesh Pati. "In vitro clonal propagation of bael (Aegle marmelos Corr.) CV. CISHB1 through enhanced axillary branching" Physiol. Mol. Biol. Plants Vol. 14 Iss. 4 (2008)
Available at: http://works.bepress.com/rajesh_pati/9/