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Cadherin-2 Participates in the Morphogenesis of the Zebrafish Inner Ear
Journal of Cell Science (2006)
  • Sherry Babb-Clendenon, Indiana University
  • Yu-chi Shen, University of Michigan - Ann Arbor
  • Qin Liu, University of Akron
  • Katharyn E. Turner, Indiana University
  • M. Susan Mills, Indiana University
  • Greg W. Cook, Indiana University
  • Caroline A. Miller, Indiana University
  • Vincent H. Gattone, II, Indiana University
  • Kate F. Barald, University of Michigan - Ann Arbor
  • James A. Marrs, Indiana University

Molecular mechanisms that control inner ear morphogenesis from the placode to the three-dimensional functional organ are not well understood. We hypothesize that cell-cell adhesion, mediated by cadherin molecules, contributes significantly to various stages of inner ear formation. Cadherin-2 (Cdh2) function during otic vesicle morphogenesis was investigated by examining morpholino antisense oligonucleotide knockdown and glass onion (glo) ( Cdh2 mutant) zebrafish embryos. Placode formation, vesicle cavitation and specification occurred normally, but morphogenesis of the otic vesicle was affected by Cdh2 deficiency: semicircular canals were reduced or absent. Phalloidin staining of the hair cell stereocillia demonstrated that cadherin-2 (cdh2) loss-of-function did not affect hair cell number, but acetylated tubulin labeling showed that hair cell kinocilia were shorter and irregularly shaped. Statoacoustic ganglion size was significantly reduced, which suggested that neuron differentiation or maturation was affected. Furthermore, cdh2 loss-of-function did not cause a general developmental delay, since differentiation of other tissues, including eye, proceeded normally. These findings demonstrate that Cdh2 selectively affects epithelial morphogenetic cell movements, particularly semicircular canal formation, during normal ear mophogenesis.

  • N-cadherin,
  • Zebrafish,
  • Ear development,
  • Morphogenesis,
  • Antisense oligonucleotide
Publication Date
December 15, 2006
Publisher Statement
The original published version of this article may be found at
Citation Information
Sherry Babb-Clendenon, Yu-chi Shen, Qin Liu, Katharyn E. Turner, et al.. "Cadherin-2 Participates in the Morphogenesis of the Zebrafish Inner Ear" Journal of Cell Science Vol. 119 Iss. 24 (2006)
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