Skip to main content
Novel ceramic bone replacement material CeraBall® seeded with human mesenchymal stem cells
Clinical oral implants research
  • Timothy Douglas, University of Kiel
  • Qin Liu, University of Kiel
  • Andreas Humpe, University Hospital Schleswig-Holstein
  • Jörg Wiltfang, University of Kiel
  • Sureshan Sivananthan, University of London
  • Patrick H. Warnke, Bond University
Date of this Version
Document Type
Journal Article
Publication Details

Interim status: Citation only.

Douglas, T., Liu, Q., Humpe, A., Wiltfang, J., Sivananthan, S., & Warnke, P. H. (2010). Novel ceramic bone replacement material CeraBall® seeded with human mesenchymal stem cells, Clinical Oral Implants Research, 21(3), 262-267.

Access the Journal's homepage.

2010 HERDC submission. FoR Code: 100404, 110504

© Copyright 2009 John Wiley & Sons A/S

Objectives: Hydroxyapatite (HA) and tricalcium phosphate (TCP) are two very common ceramic materials for bone replacement. A recently developed material for bone replacement is CeraBall®, which is a mixed HA-TCP scaffold available as porous spherical scaffolds of diameter 4 and 6 mm. Before their use as bone replacement materials in vivo, in vitro testing of these scaffolds is necessary. The goal of this study was to characterise 4 and 6 mm CeraBall® scaffolds in vitro with a view to their future use as bone replacement materials. Materials and methods: The proliferation of human mesenchymal stromal cells (hMSCs) seeded on CeraBall® scaffolds was evaluated quantitatively using the WST [Water soluble tetrazolium ((4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1, 3-benzene disulfonate)] test and qualitatively by scanning electron microscopy (SEM). In addition, the standard MTT [(3-(4, 5-Dimenthylthiazol-2-Y1)-2, 5-Diphenyltetrazolium bromide)] biocompatibility test and cell vitality staining were performed using hMSCs. CeraBall® scaffolds were also tested for their mechanical properties. Results: SEM and WST test results showed that hMSCs proliferated on CeraBall® scaffolds over the course of 9 days. Proliferation was similar to that seen on tissue culture polystyrene (control). Cells showed a well-spread morphology and formed 'sheets' on the surface of scaffolds. Invasion of pores was observed. Good biocompatibility was demonstrated by MTT test results and cell vitality staining. Scaffolds of both 4 and 6 mm were able to withstand compressive loads of 5 N. Conclusions: CeraBall® scaffolds show good biocompatibility in vitro for hMSCs. This opens the way for in vivo applications.
Citation Information
Timothy Douglas, Qin Liu, Andreas Humpe, Jörg Wiltfang, et al.. "Novel ceramic bone replacement material CeraBall® seeded with human mesenchymal stem cells" Clinical oral implants research Vol. 21 Iss. 3 (2010) p. 262 - 267
Available at: