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Adaptive mechanisms of Campylobacter jejuni to erythromycin treatment
BMC Microbiology
  • Qingqing Xia, Iowa State University
  • Wayne T. Muraoka, Iowa State University
  • Zhangqi Shen, Iowa State University
  • Orhan Sahin, Iowa State University
  • Hongning Wang, Sichuan University
  • Zuowei Wu, Iowa State University
  • Peng Liu, Iowa State University
  • Qijing Zhang, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
6-14-2013
DOI
10.1186/1471-2180-13-133
Abstract
Background Macrolide is the drug of choice to treat human campylobacteriosis, but Campylobacter resistance to this antibiotic is rising. The mechanisms employed by Campylobacter jejuni to adapt to erythromycin treatment remain unknown and are examined in this study. The transcriptomic response of C. jejuni NCTC 11168 to erythromycin (Ery) treatment was determined by competitive microarray hybridizations. Representative genes identified to be differentially expressed were further characterized by constructing mutants and assessing their involvement in antimicrobial susceptibility, oxidative stress tolerance, and chicken colonization. ResultsFollowing the treatment with an inhibitory dose of Ery, 139 genes were up-regulated and 119 were down-regulated. Many genes associated with flagellar biosynthesis and motility was up-regulated, while many genes involved in tricarboxylic acid cycle, electron transport, and ribonucleotide biosynthesis were down-regulated. Exposure to a sub-inhibitory dose of Ery resulted in differential expression of much fewer genes. Interestingly, two putative drug efflux operons (cj0309c-cj0310c and cj1173-cj1174) were up-regulated. Although mutation of the two operons did not alter the susceptibility of C. jejuni to antimicrobials, it reduced Campylobacter growth under high-level oxygen. Another notable finding is the consistent up-regulation of cj1169c-cj1170c, of which cj1170c encodes a known phosphokinase, an important regulatory protein in C. jejuni. Mutation of the cj1169c-cj1170c rendered C. jejuni less tolerant to atmospheric oxygen and reduced Campylobacter colonization and transmission in chickens. Conclusions These findings indicate that Ery treatment elicits a range of changes in C. jejuni transcriptome and affects the expression of genes important for in vitro and in vivo adaptation. Up-regulation of motility and down-regulation of energy metabolism likely facilitate Campylobacter to survive during Ery treatment. These findings provide new insight into Campylobacter adaptive response to antibiotic treatment and may help to understand the mechanisms underlying antibiotic resistance development.
Comments

This article is from BMC Microbiology 13 (2013): 133, doi:10.1186/1471-2180-13-133. Posted with permission.

Rights
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright Owner
Xia et al
Language
en
File Format
application/pdf
Citation Information
Qingqing Xia, Wayne T. Muraoka, Zhangqi Shen, Orhan Sahin, et al.. "Adaptive mechanisms of Campylobacter jejuni to erythromycin treatment" BMC Microbiology Vol. 13 (2013) p. 133
Available at: http://works.bepress.com/qijing-zhang/8/