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Molecular Evidence for Zoonotic Transmission of an Emergent, Highly Pathogenic Campylobacter jejuni Clone in the United States
Journal of Clinical Microbiology
  • Orhan Sahin, Iowa State University
  • Collette Fitzgerald, Iowa State University
  • Steven Stroika, Iowa State University
  • Shaohua Zhao, Food and Drug Administration
  • Rachel Sippy, Iowa State University
  • Patrick Kwan, Centers for Disease Control and Prevention
  • Paul J. Plummer, Iowa State University
  • Jing Han, Iowa State University
  • Michael Yaeger, Iowa State University
  • Qijing Zhang, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
3-1-2012
DOI
10.1128/JCM.06167-11
Abstract
Campylobacter jejuni is a major zoonotic pathogen. A highly virulent, tetracycline-resistant C. jejuni clone (clone SA) has recently emerged in ruminant reservoirs and has become the predominant cause of sheep abortion in the United States. To determine whether clone SA is associated with human disease, we compared the clinical isolates of clone SA from sheep abortions with the human isolates of the PulseNet National Campylobacter databases at the CDC and the FDA using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and serotyping. The combined SmaI and KpnI PFGE pattern designations of clone SA from sheep were indistinguishable from those of 123 (9.03%) human C. jejuniisolates (total, 1,361) in the CDC database, among which 56 were associated with sporadic infections and 67 were associated with outbreaks that occurred in multiple states from 2003 to 2010. Most of the outbreaks were attributed to raw milk, while the sources for most of the sporadic cases were unknown. All clone SA isolates examined, including PFGE-matched human isolates, belong to sequence type 8 (ST-8) by MLST and serotype HS:1,8, further indicating the clonality of the related isolates from different host species. Additionally, C. jejuni clone SA was identified in raw milk, cattle feces, the feces and bile of healthy sheep, and abortion cases of cattle and goats, indicating the broad distribution of this pathogenic clone in ruminants. These results provide strong molecular and epidemiological evidence for zoonotic transmission of this emergent clone from ruminants to humans and indicate that C. jejuni clone SA is an important threat to public health.
Comments

This article is from Journal of Clinical Microbiology 50 (2012): 680, doi:10.1128/JCM.06167-11.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Orhan Sahin, Collette Fitzgerald, Steven Stroika, Shaohua Zhao, et al.. "Molecular Evidence for Zoonotic Transmission of an Emergent, Highly Pathogenic Campylobacter jejuni Clone in the United States" Journal of Clinical Microbiology Vol. 50 Iss. 3 (2012) p. 680 - 687
Available at: http://works.bepress.com/qijing-zhang/13/