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Article
A PCR Assay for Specific Detection of the Pandemic Vibrio parahaemolyticus O3:K6 Clone from Shellfish
Journal of Food Science
  • P.S. Marie Yeung, Cornell University
  • A. DePaola, Cornell University
  • C. A. Kaysner, Gulf Coast Seafood Laboratory- Food and Drug Administration
  • K. J. Boor, Seafood Products Research Center - Food and Drug Administration
Publication Date
5-1-2003
Abstract

The current standard method for identifying Vibrio parahaemolyticus serotype O3:K6, an emerging pathogen with apparent enhanced virulence characteristics, typically takes 4 to 6 d to complete and requires serotyping. To provide a more rapid strategy, we optimized a polymerase chain reaction (PCR)-based assay for specific detection of V. parahaemolyticus O3:K6. Of 78 V. parahaemolyticus isolates and other related species; only strains classified into the V. parahaemolyticus O3:K6 clonal group (n = 39) showed positive results in the PCR assay. The assay detected 2.3 cells/PCR reaction and 310 cells/g using bacterial cultures and inoculated oyster samples, respectively. Sensitive and specific detection of V. parahaemolyticus O3:K6 was possible following a 6-h enrichment.

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Citation Information
P.S. Marie Yeung, A. DePaola, C. A. Kaysner and K. J. Boor. "A PCR Assay for Specific Detection of the Pandemic Vibrio parahaemolyticus O3:K6 Clone from Shellfish" Journal of Food Science Vol. 68 Iss. 4 (2003) p. 1459 - 1466
Available at: http://works.bepress.com/pmyeung/7/