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Article
Application of Genotypic and Phenotypic Analyses to Commerical Probiotic Strain Identity and Relatedness
Journal of Applied Microbiology
  • P.S.M. Yeung, California Polytechnic State University - San Luis Obispo
  • C. L. Kitts, California Polytechnic State University - San Luis Obispo
  • R. Cano, California Polytechnic State University - San Luis Obispo
  • P. Tong, California Polytechnic State University - San Luis Obispo
Publication Date
11-1-2004
Abstract

Aims: The objective of this study was to generate strain-specific genomic patterns of a bank of 67 commercial and reference probiotic strains, with a focus on probiotic lactobacilli.

Methods and Results: Pulsed-field gel electrophoresis (PFGE) was used as the primary method for strain differentiation. This method was compared with carbohydrate fermentation analysis. To supplement visual comparison, PFGE patterns were analysed quantitatively by cluster analysis using unweighted pair group method with arithmetic averages. SmaI, NotI and XbaI were found to effectively generate clear and easy-to-interpret PFGE patterns of a range of probiotic strains. Some probiotic strains from different sources shared highly similar PFGE patterns.

Conclusions: Results document the value of genotypic strain identification methods, combined with phenotypic methods, for determining probiotic strain identity and relatedness. No correlation was found between relatedness determined by carbohydrate fermentation profiles alone compared with PFGE analysis alone. Some commercial strains are probably derived from similar sources.

Significance and Impact of the Study: This approach is valuable to the probiotic industry to develop commercial strain identification patterns, to provide quality control of strain manufacturing production runs, to track use of protected strains and to determine the relatedness among different research and commercial probiotic strains.

Disciplines
Publisher statement
The Definitive version is available at www.blackwell-synergy.com
Citation Information
P.S.M. Yeung, C. L. Kitts, R. Cano and P. Tong. "Application of Genotypic and Phenotypic Analyses to Commerical Probiotic Strain Identity and Relatedness" Journal of Applied Microbiology Vol. 97 Iss. 5 (2004) p. 1095 - 1104
Available at: http://works.bepress.com/pmyeung/3/