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Structural classification of biotin carboxyl carrier proteins
Biotechnology Letters
  • Yingfei Chen, Iowa State University
  • Armando Elizondo-Noriega, Iowa State University
  • David C. Cantu, Iowa State University
  • Peter J. Reilly, Iowa State University
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We gathered primary and tertiary structures of acyl-CoA carboxylases from public databases, and established that members of their biotin carboxylase (BC) and biotin carboxyl carrier protein (BCCP) domains occur in one family each and that members of their carboxyl transferase (CT) domains occur in two families. Protein families have members similar in primary and tertiary structure that probably have descended from the same protein ancestor. The BCCP domains complexed with biotin in acyl and acyl-CoA carboxylases transfer bicarbonate ions from BC domains to CT domains, enabling the latter to carboxylate acyl and acyl-CoA moieties. We separated the BCCP domains into four subfamilies based on more subtle primary structure differences. Members of different BCCP subfamilies often are produced by different types of organisms and are associated with different carboxylases.

This is a post-print of an article from Biotechnology Letters, 34, no. 10 (October 2012): 1869–1875, doi: 10.1007/s10529-012-0978-4.

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Springer Science+Business Media B.V.
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Yingfei Chen, Armando Elizondo-Noriega, David C. Cantu and Peter J. Reilly. "Structural classification of biotin carboxyl carrier proteins" Biotechnology Letters Vol. 34 Iss. 10 (2012) p. 1869 - 1875
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