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Article
Temporal evolution of gene expression in rat carotid artery following balloon angioplasty
Odgren Lab
  • Jian-ming Li, University of Massachusetts Medical School
  • Xueqing Zhang, University of Massachusetts Medical School
  • Peter R. Nelson, University of Florida College of Medicine
  • Paul R. Odgren, University of Massachusetts Medical School
  • Janice D. Nelson, University of Florida College of Medicine
  • Calin A. Vasiliu, UMass Memorial Health Care
  • Jane Park, University of Massachusetts Medical School
  • Marvin Morris, University of Massachusetts Medical School
  • Jane B. Lian, University of Massachusetts Medical School
  • Bruce S. Cutler, University of Massachusetts Medical School
  • Peter E. Newburger, University of Massachusetts Medical School
UMMS Affiliation
Department of Surgery; Department of Cell Biology; Department of Pediatrics
Date
5-15-2007
Document Type
Article
Medical Subject Headings
*Angioplasty, Balloon; Animals; *Carotid Arteries; Computational Biology; Gene Expression Profiling; *Gene Expression Regulation; Hyperplasia; In Situ Hybridization; Intercellular Signaling Peptides and Proteins; Male; Oligonucleotide Array Sequence Analysis; Oxidative Stress; Rats; Rats, Sprague-Dawley
Disciplines
Abstract
The success of vascular intervention including angioplasty, stenting, and arterial bypass remains limited by negative remodeling resulted in lumen restenosis. This study was to characterize the global transcription profile reflecting concurrent events along arterial remodeling and neointima formation in a rat carotid artery balloon-injury model. Expression profiling of injured and control common carotid arteries on days 4, 7, 14 post-injury that mark the major pathohistological progression stages of neointimal formation were recorded on high-density oligonucleotide arrays. A subset of genes from microarray-based data was further studied using quantitative real time RT-PCR and in situ hybridization with sequential arterial samples from days 1 to 28 post-injury. The gene-encoded proteins were validated with Western blot. Besides temporal induction of a large cluster of genes over-represented by cell proliferation and macromolecule metabolism gene ontology categories, a fast-evolving inflammation could be demonstrated by the induction of Tgfb and other anti-inflammatory genes (e.g., C1qtnf3 (C1q and tumor necrosis factor related protein 3 (predicted))) and a shift from type 1 to 2 helper T cell response. The most significant signature of the induced neointimal profile is enrichment of genes functionally related to angiogenesis and extracellular matrix (ECM) remodeling (e.g., Spp1 (secreted phosphoprotein 1), CD44 (CD44 antigen), and Cxcl12 (chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)). Some of the genes represent stress-responsive mesenchymal stromal cell cytokines. This study highlighted mesenchymal stromal cell cytokines-driven inflammatory extracellular matrix remodeling, as target processes for potential clinical therapeutic intervention.
Rights and Permissions
Citation: J Cell Biochem. 2007 May 15;101(2):399-410. Link to article on publisher's site
Related Resources
Link to Article in PubMed
PubMed ID
17171642
Citation Information
Jian-ming Li, Xueqing Zhang, Peter R. Nelson, Paul R. Odgren, et al.. "Temporal evolution of gene expression in rat carotid artery following balloon angioplasty" Vol. 101 Iss. 2 (2007) ISSN: 0730-2312 (Linking)
Available at: http://works.bepress.com/paul_odgren/25/