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SPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid activation signaling pathway in C. elegans
BMC Genetics (2014)
  • Paul J Muhlrad, University of Colorado Boulder
  • Jessica N Clark
  • Ubaydah Nasri
  • Nicholas G Sullivan
  • Craig W LaMunyon
Abstract
Here, we identify SPE-8 as a member of a large family of sperm-expressed non-receptor-like protein-tyrosine kinases. A rescuing SPE-8::GFP translational fusion reporter localizes to the plasma membrane in all spermatogenic cells from the primary spermatocyte stage through spermatids. Once spermatids become activated to spermatozoa, the reporter moves from the plasma membrane to the cytoplasm. Mutations in the spe-8 group genes spe-12, spe-19, and spe-27 disrupt localization of the reporter to the plasma membrane, while localization appears near normal in a spe-29 mutant background.
Keywords
  • Caenorhabditis elegans,
  • Spermatogenesis,
  • Sperm activation,
  • spe-8,
  • Signal transduction
Publication Date
July 14, 2014
Citation Information
Paul J Muhlrad, Jessica N Clark, Ubaydah Nasri, Nicholas G Sullivan, et al.. "SPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid activation signaling pathway in C. elegans" BMC Genetics Vol. 15 Iss. 83 (2014)
Available at: http://works.bepress.com/paul_muhlrad/4/