Objective: Evaluate physiologic changes during digital retinal imaging (DRI) using near infra-red spectroscopy (NIRS).

Study design: Prospective observational study of preterm infants undergoing retinopathy of prematurity screening via DRI using wide-field retinal camera. Cardiorespiratory (CR) and NIRS data were collected, trends correlated for changes and coefficient representing "slopes" of outcomes were plotted over time. The p value associated with each slope coefficient was tested to assess for slope differences from time of intervention (time = 0/or no slope).

Results: Thirty-one preterm infants were included in the study. There were no significant changes in pre- and post-slopes for cerebral or mesenteric oxygenation, or CR indices with eye drop administration compared to baseline. DRI resulted in significant increase in post exam slope in cerebral oxygenation, mesenteric oxygenation and respiratory rate.

Conclusion: ROP examination using DRI was well tolerated with slight improvements in cerebral and mesenteric perfusion without significant safety concerns.