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A Pectin Methylesterase ZmPme3 Is Expressed in Gametophyte factor1-s (Ga1-s) Silks and Maps to that Locus in Maize (Zea mays L.)
Frontiers in Plant Science
  • Adrienne N. Moran Lauter, U.S. Department of Agriculture
  • Michael G. Muszynski, University of Hawaii, Manoa
  • Ryan D. Huffman, Iowa State University
  • M. Paul Scott, U.S. Department of Agriculture
Document Type
Article
Publication Version
Published Version
Publication Date
11-1-2017
DOI
10.3389/fpls.2017.01926
Abstract
The ga1 locus of maize confers unilateral cross incompatibility, preventing cross pollination between females carrying the incompatible Ga1-s allele and males not carrying a corresponding compatible allele. To characterize this system at the molecular level, we carried out a transcript profiling experiment in which silks from near isogenic lines carrying the Ga1-sand ga1 alleles were compared. While several differentially expressed genes were identified, only one mapped to the known location of ga1. This gene is a pectin methylesterase (PME), which we designated as ZmPme3, and is present and expressed only in Ga1-s genotypes. While a functional ZmPME3 is not present in the ga1 genotypes examined, a pectin methylesterase gene cluster is found in ga1 genotypes. The gene cluster in W22 contains 58 tandem full-length or partial PME pseudo genes. These data combined with a wealth of previously published data on the involvement of PMEs in pollen tube growth suggest a role for cell wall modification enzymes in the pollen exclusion component of Ga1-s gametophytic incompatibility. Consistent with this role, a third allele which lacks the female function of Ga1-s, Ga1-m, has a mutationally inactivated version of ZmPme3.
Comments

This article is published as Moran Lauter AN, Muszynski MG, Huffman RD and Scott MP (2017) A Pectin Methylesterase ZmPme3 Is Expressed in Gametophyte factor1-s (Ga1-s) Silks and Maps to that Locus in Maize (Zea mays L.). Front. Plant Sci. 8:1926. doi: 10.3389/fpls.2017.01926.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Adrienne N. Moran Lauter, Michael G. Muszynski, Ryan D. Huffman and M. Paul Scott. "A Pectin Methylesterase ZmPme3 Is Expressed in Gametophyte factor1-s (Ga1-s) Silks and Maps to that Locus in Maize (Zea mays L.)" Frontiers in Plant Science Vol. 8 (2017) p. 1926
Available at: http://works.bepress.com/paul-scott/94/