Skip to main content
Contribution to Book
Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds
Methods in Molecular Biology: Transgenic Maize
  • M. Paul Scott, United States Department of Agriculture
Document Type
Book Chapter
Publication Version
Published Version
Publication Date
1-1-2009
DOI
10.1007/978-1-59745-494-0_10
Abstract
This method allows high-throughput qualitative screening to identify seeds containing a transgene product in endosperm tissue. It is particularly useful for determining genetic segregation ratios or identifying seeds to be advanced in a breeding program. Tissue printing is used to avoid time-consuming extraction steps. Antibody-based detection of the transgene product makes this method suitable to any transgene product for which a specific antibody is available. It is possible to screen thousands of seeds per week using this method.
Comments

This chapter is from Scott, M. Paul. "Tissue-print immunodetection of transgene products in endosperm for high-throughput screening of seeds." Transgenic Maize: Methods and Protocols (2009): 123-128, doi: 10.1007/978-1-59745-494-0_10.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
M. Paul Scott. "Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds" Methods in Molecular Biology: Transgenic Maize Vol. 526 (2009) p. 123 - 128
Available at: http://works.bepress.com/paul-scott/88/