Contribution to Book
Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of SeedsMethods in Molecular Biology: Transgenic Maize
Document TypeBook Chapter
Publication VersionPublished Version
AbstractThis method allows high-throughput qualitative screening to identify seeds containing a transgene product in endosperm tissue. It is particularly useful for determining genetic segregation ratios or identifying seeds to be advanced in a breeding program. Tissue printing is used to avoid time-consuming extraction steps. Antibody-based detection of the transgene product makes this method suitable to any transgene product for which a specific antibody is available. It is possible to screen thousands of seeds per week using this method.
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Citation InformationM. Paul Scott. "Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds" Methods in Molecular Biology: Transgenic Maize Vol. 526 (2009) p. 123 - 128
Available at: http://works.bepress.com/paul-scott/88/