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Article
Changes in endogenous gene transcript and protein levels in maize plants expressing the soybean ferritin transgene
Frontiers in Plant Science
  • Milly N. Konobe, Iowa State University
  • Steven R. Rodermel, Iowa State University
  • Theodore Bailey, Iowa State University
  • M. Paul Scott, United States Department of Agriculture
Document Type
Article
Publication Version
Published Version
Publication Date
6-14-2013
DOI
10.3389/fpls.2013.00196
Abstract
Transgenic agricultural crops with increased nutritive value present prospects for contributing to public health. However, their acceptance is poor in many countries due to the perception that genetic modification may cause unintended effects on expression of native genes in the host plant. Here, we tested effects of soybean ferritin transgene (SoyFer1, M64337) on transcript and protein levels of endogenous genes in maize. Results showed that the transgene was successfully introduced and expressed in the maize seed endosperm. mRNA abundance of seven tested iron homeostasis genes and seed storage protein genes differed significantly between seed samples positive and negative for the transgene. The PCR negative samples had higher zein and total protein content compared to the positive samples. However, PCR positive samples had significantly higher concentrations of calcium, magnesium, and iron. We have shown that the soybean ferritin transgene affected the expression of native iron homeostasis genes in the maize plant. These results underscore the importance of taking a holistic approach to the evaluation of transgenic events in target plants, comparing the transgenic plant to the untransformed controls.
Comments

This article is published as Kanobe, Milly N., Steven R. Rodermel, Theodore Bailey, and Paul Scott. "Changes in endogenous gene transcript and protein levels in maize plants expressing the soybean ferritin transgene." Frontiers in Plant Science 4 (2013): 196, doi: 10.3389/fpls.2013.00196.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Milly N. Konobe, Steven R. Rodermel, Theodore Bailey and M. Paul Scott. "Changes in endogenous gene transcript and protein levels in maize plants expressing the soybean ferritin transgene" Frontiers in Plant Science Vol. 4 (2013) p. 196
Available at: http://works.bepress.com/paul-scott/68/