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Carbon isotope ratios of amylose, amylopectin and mutant starches
  • M. Paul Scott, United States Department of Agriculture
  • Jay-Lin Jane, Iowa State University
  • Madhavan Soundararajan, University of Nebraska - Lincoln
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Carbon isotope ratios (expressed as δ13C values) were determined for various sources of starch and the starch fractions amylose and amylopectin. The δ13C values of amylose were consistently less negative, 0.4–2.3 ‰, than those of amylopectin in kernal starch from maize (Zea mays) and barley (Hordeum vulgare) and in tuber starch from potato (Solanum tuberosum). Kernel starch isolated from the maize mutants wx1 and ae1, with known genetic lesions in the starch biosynthetic pathway, also showed significant differences in δ13C values. Collectively, these results suggest that variation in carbon isotope ratios in the amylose and amylopectin components of starch may be attributed to isotopic discrimination by the enzymes involved in starch biosynthesis.


This article is published as Scott, M. Paul, Jay-Lin Jane, and Madhavan Soundararajan. "Carbon isotope ratios of amylose, amylopectin and mutant starches." Phytochemistry 52, no. 4 (1999): 555-559, doi: 10.1016/S0031-9422(99)00271-X.

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M. Paul Scott, Jay-Lin Jane and Madhavan Soundararajan. "Carbon isotope ratios of amylose, amylopectin and mutant starches" Phytochemistry Vol. 52 Iss. 4 (1999) p. 555 - 559
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