Article
Detection of duck enteritis virus by polymerase chain reaction
Avian Diseases
(1998)
Abstract
SUMMARY. Duck enteritis virus (DEV), a herpesvirus, is the causative agent of duck
viral enteritis in free-flying, feral, and domesticated members of the Anatidae family. HindIIIldigested
DEV DNA was cloned into the plasmid pBluescript, and a 1.95-kb fragment was
sequenced. This fragment codes for the 3' region of the DEV homologues of varicella-zoster
virus (VZV) open reading frame (ORF) UL6 and the 5' region of VZV UL7. Alignment of
the putative peptide fragments for DEV UL6 and UL7 showed a 64% and 37% identity
with VZV UL6 and UL7, respectively. Primers located in the highly conserved domain of
the UL6 gene were used for a polymerase chain reaction (PCR) assay, which was able to
amplify DEV DNA. The PCR assay also amplified DEV DNA from the original outbreak
samples and/or after passage in Muscovy duck embryos.
Disciplines
Publication Date
1998
DOI
10.2307/1592682
Publisher Statement
This article is from Avian Diseases 42 (1998): 554, doi:10.2307/1592682. Posted with permission.
Copyright 2015 American Association of Avian Pathologists
Citation Information
Paul J. Plummer, Tim Alefantis, Steven Kaplan, Priscilla O'Connell, et al.. "Detection of duck enteritis virus by polymerase chain reaction" Avian Diseases Vol. 42 Iss. 3 (1998) p. 554 - 564 Available at: http://works.bepress.com/paul-plummer/19/