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Serum Concentrations of Haptoglobin and Haptoglobin-Matrix Metalloproteinase 9 (Hp-MMP 9) Complexes of Bovine Calves in a Bacterial Respiratory Challenge Model
BMC Veterinary Research
  • Christy Jo Hanthorn, Iowa State University
  • Grant A. Dewell, Iowa State University
  • Reneé Dewell, Iowa State University
  • Vickie L. Cooper, Iowa State University
  • Chong Wang, Iowa State University
  • Paul J. Plummer, Iowa State University
  • Jeff Lakritz, Ohio State University
Document Type
Article
Publication Version
Published Version
Publication Date
12-1-2014
DOI
10.1186/s12917-014-0285-5
Abstract
Serum haptoglobin (Hp) and haptoglobin matrix metalloproteinase 9 complexes (Hp-MMP 9) have been identified as biomarkers with diagnostic potential in cattle with conditions resulting in an acute inflammatory response. The purpose of this study was to evaluate potential diagnostic applications of serum Hp and Hp-MMP 9 concentrations in calves with BRD and establish a timeline for their detection in calves experimentally challenged with Bibersteinia trehalosi and Mannheimia haemolytica. Thirty-five cross bred dairy calves were inoculated via tracheal catheterization with either a PCR confirmed leukotoxin negative B. trehalosi isolate, a PCR confirmed leukotoxin positive B. trehalosi isolate, a Mannheimia haemolyticaisolate, a combination of leukotoxin negative B. trehalosi and M. haemolytica, or a negative control. Serum samples were collected throughout the study. Calves were euthanized and necropsy performed on day 10 post inoculation. M. haemolytica inoculated calves had increased lung involvement. Serum Hp and Hp- MMP 9 concentrations were elevated compared to the other treatment groups. Increases in serum Hp and Hp-MMP 9 concentrations for the M. haemolyticagroup were significantly different from other study groups on day 7 of the study. B. trehalosi inoculated calves did not have increased lung involvement compared to control calves, but the leukotoxin positive B. trehalosi group demonstrated increased serum Hp-MMP 9 concentrations from day 3 to the end of the study compared to the pre-inoculation concentrations. Serum Hp-MMP 9 concentration is a useful diagnostic tool for detecting early pulmonary inflammation in calves challenged with B. trehalosi and M. haemolytica. Serum Hp-MMP 9 may also be a useful tool in detecting subclinical pulmonary inflammation in challenged calves.
Comments

This article is from BMC Veterinary Research 10 (2014): 285, doi:10.1186/s12917-014-0285-5. Posted with permission.

Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright Owner
Christy J. Hanthorn, et al
Language
en
File Format
application/pdf
Citation Information
Christy Jo Hanthorn, Grant A. Dewell, Reneé Dewell, Vickie L. Cooper, et al.. "Serum Concentrations of Haptoglobin and Haptoglobin-Matrix Metalloproteinase 9 (Hp-MMP 9) Complexes of Bovine Calves in a Bacterial Respiratory Challenge Model" BMC Veterinary Research Vol. 10 (2014) p. 1 - 7
Available at: http://works.bepress.com/paul-plummer/17/