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Dihydrolipoic Acid Conjugated Carbon Dots Accelerate Human Insulin Fibrillation
Journal of Parkinson's Disease and Alzheimer's Disease
  • Sheba J. Kuruvilla, University of Miami
  • Shanghao Li, University of Miami
  • Lorenzo Sansalone, University of Miami
  • Blake Fortes, University of Miami
  • Ian Zheng, University of Miami
  • Patricia Blackwelder, University of Miami; Nova Southeastern University
  • Cyrus Pumilia, University of Miami
  • Miodrag Micic, Cerritos College; MP Biomedicals LLC
  • Jhony Orbulescu, MP Biomedicals LLC
  • Roger M. Leblanc, University of Miami
Document Type
Publication Date
  • Protein fibrillation,
  • Insulin,
  • Carbon dots,
  • Nanoparticles
Protein fibrillation is believed to play an important role in the pathology and development of several human diseases, such as Alzheimer’s disease, Parkinson’s disease and type 2 diabetes. Carbon dots (CDs), as a new type of nanoparticle have recently been extensively studied for potential biological applications, but their effects on protein fibrillation remain unexplored. In reality, any application in biological systems will inevitably have “contact” between proteins and CDs. In this study, human insulin was selected as a model protein to study the effects of CDs on protein fibrillation, as proteins may share a common mechanism to form fibrils. Hydrophobic CDs were conjugated with dihydrolipoic acid (DHLA-CDs) to facilitate their water solubility. Characterizations from thioflavin T fluorescence, circular dichroism spectroscopy and atomic force microscopy demonstrate that the presence of DHLA-CDs results in a higher rate of human insulin fibrillation, accelerating the conformational changes of human insulin from α-helix to β-sheet. This promoting effect is likely associated with the locally increased concentration of human insulin adsorbed on the surface of DHLA-CDs.

©2015 Leblanc et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Additional Comments
NSF grant #: 1355317
Citation Information
Sheba J. Kuruvilla, Shanghao Li, Lorenzo Sansalone, Blake Fortes, et al.. "Dihydrolipoic Acid Conjugated Carbon Dots Accelerate Human Insulin Fibrillation" Journal of Parkinson's Disease and Alzheimer's Disease Vol. 2 Iss. 1 (2015) p. 1 - 7 ISSN: 2376-922X
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