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Article
Florida Reef Sponges Harbor Coral Disease-Associated Microbes
Symbiosis
  • Karita Negandhi, Nova Southeastern University
  • Patricia Blackwelder, Nova Southeastern University
  • Alexander V. Ereskovsky, Saint-Petersburg State University
  • Jose V Lopez, Nova Southeastern University
Document Type
Article
Publication Date
7-1-2010
Keywords
  • Sponge,
  • Coral Disease,
  • Bacteria,
  • FISH,
  • Spatial Arrangement
Peer Reviewed
1
Abstract
Sponges can filter large volumes of seawater and accumulate highly diverse and abundant microbial communities within their tissue. Culture-independent techniques such as fluorescent in situ hybridization (FISH), 16S small subunit (SSU) rRNA gene analyses, and transmission electron microscopy (TEM) were applied to characterize the presence and distribution of microbes within sponges abundant on south Florida reefs. This study found that coral disease-associated bacteria (CDAB) are harbored within Agelas tubulata andAmphimedon compressa. FISH probes detected several potential bacterial pathogens such as Aurantimonas coralicida, Cytophaga sp., Desulfovibriospp, Serratia marcescans, and Vibrio mediterranei within A. compressa andA. tubulata host sponges. Spatial differences in the distribution of targeted bacteria were seen within sponge hosts. Transmission electron microscopy ofA. compressa indicated there was a higher concentration of bacteria in the choanosome compared to the ectosome. These observed spatial distributions support the presence of internal sponge niches, which could play a role in the location of the CDAB within the sponges.
ORCID ID
0000-0002-1637-4125
ResearcherID
F-8809-2011
DOI
10.1007/s13199-010-0059-1
Citation Information
Negandhi, Karita, PatriciaL Blackwelder, AlexanderV Ereskovsky, and JoseV Lopez. 2010. "Florida reef sponges harbor coral disease-associated microbes." Symbiosis no. 51 (1):117-129. doi: 10.1007/s13199-010-0059-1.