Skip to main content
Article
Glyphosate Induces Neurotoxicity in Zebrafish
Biology Faculty Publications
  • Nicole Roy, Sacred Heart University
  • Bruno Carneiro, Sacred Heart University
  • Jeremy Ochs, Sacred Heart University
Document Type
Article
Publication Date
1-4-2016
Abstract

Glyphosate based herbicides (GBH) like Roundup® are used extensively in agriculture as well as in urban and rural settings as a broad spectrum herbicide. Its mechanism of action was thought to be specific only to plants and thus considered safe and non-toxic. However, mounting evidence suggests that GBHs may not be as safe as once thought as initial studies in frogs suggest that GBHs may be teratogenic. Here we utilize the zebrafish vertebrate model system to study early effects of glyphosate exposure using technical grade glyphosate and the Roundup® Classic formulation. We find morphological abnormalities including cephalic and eye reductions and a loss of delineated brain ventricles. Concomitant with structural changes in the developing brain, using in situ hybridization analysis, we detect decreases in genes expressed in the eye, fore and midbrain regions of the brain including pax2, pax6, otx2 and ephA4. However, we do not detect changes in hindbrain expression domains of ephA4 nor exclusive hindbrain markers krox-20 and hoxb1a. Additionally, using a Retinoic Acid (RA) mediated reporter transgenic, we detect no alterations in the RA expression domains in the hindbrain and spinal cord, but do detect a loss of expression in the retina. We conclude that glyphosate and the Roundup® formulation is developmentally toxic to the forebrain and midbrain but does not affect the hindbrain after 24 hour exposure.

Comments

The version posted is the unedited manuscript that has been accepted for publication.

PMID: 26773362

DOI
doi:10.1016/j.etap.2016.01.003
Citation Information
Roy, N. M., Carneiro, B., & Ochs, J. (2016). Glyphosate Induces Neurotoxicity in Zebrafish. Environmental Toxicology and Pharmacology. doi:10.1016/j.etap.2016.01.003