Article
Requirements of Rab5 activity in highly invasive breast cancer cell lines
American Society for Cell Biology
(2014)
Abstract
Rab5 expression in cancer has been associated with the disease progression and prognosis. We have previously shown that growth factor-directed cell invasion and migration was dependent on Rab5 activation in non-invasive breast cancer cells. However, hardly any data is available regarding the role of Rab5 in invasive cells in the presence of growth factor. In our present study, we report that the invasive and migratory properties of the highly invasive breast cancer cell line, MDAMB-231, were abrogated in cells expressing the inactive (GDP-bound) form of Rab5 irrespective of growth factor stimulation; while the invasive potential of breast cancer cell lines expressing the wild type and active (GTP-bound) form of Rab5 were noticeably greater. Interestingly, expression of Rin1, a Rab5 GEF, increases invasion and migration of MDAMB-231 cells even in the absence of growth factors. In contrast, the mutant Rin1 partially mitigates both processes. Hence, it is reasonable to speculate that cell invasion and migration, a process that typically involves activation of growth factor receptor, can be intracellularly regulated without intervention of activation of growth factor receptor.
Keywords
- GTPases,
- breast cancer,
- netastasis,
- invasion,
- endocytosis,
- growth factor
Disciplines
Publication Date
Winter 2014
Citation Information
Nicole Porther and M Alejandro Barbieri. "Requirements of Rab5 activity in highly invasive breast cancer cell lines" American Society for Cell Biology (2014) Available at: http://works.bepress.com/nicole_porther_chang/16/