Insulin-like growth factor-I induces migration and invasion via Rab5 ActivationCancer Biology and Endocytosis (2016)
Cell invasion, proliferation and migration are classic indicators of metastasis. Growth
factors, while important for normal cellular processes, are also pro-metastatic. In this study,
it was determined that invasion and migration in non-invasive MCF7 cells was regulated
by growth factors via Rab GTPases. It was also indicated that the invasive and migratory
properties of breast cancer MCF7 cells upon growth factor stimulation were abrogated in
cell lines that only expressed the inactive (GDP-bound) form of Rab5 irrespective of
growth factor stimulation. The invasive potential of breast cancer MCF7 cell lines
expressing the Rab5WT and active (GTP-bound) form of Rab5 mutant (Rab5:Q79L) were
noticeably greater when exposed to growth factors. In addition, we observed that Rab5 was
activated in cells incubated with IGF-1 in a time and concentration dependent manner.
Therefore, it can be concluded that Rab5 activation plays a crucial role in cell invasion,
proliferation, migration particularly in response to growth factor stimulus.
Citation InformationNicole Porther and M Alejandro Barbieri. "Insulin-like growth factor-I induces migration and invasion via Rab5 Activation" Cancer Biology and Endocytosis (2016)
Available at: http://works.bepress.com/nicole-porther/3/