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Article
Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis
Faculty of Science - Papers (Archive)
  • Dannon J. Stigers, Australian National University
  • Zachary I. Watts, Australian National University
  • James E. Hennessy, Australian National University
  • Hye-Kyung Kim, Australian National University
  • Romeo Martini, Australian National University
  • Matthew C. Taylor, CSIRO Entomology
  • Kiyoshi Ozawa, University of Wollongong
  • Jeffrey W. Keillor, Australian National University
  • Nicholas E Dixon, University of Wollongong
  • Christopher J. Easton, Australian National University
RIS ID
35338
Publication Date
1-1-2011
Publication Details

Stigers, D. J., Watts, Z. I., Hennessy, J. E., Kim, H., Martini, R., Taylor, M. C., Ozawa, K., Keillor, J. W., Dixon, N. E. & Easton, C. (2011). Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis. Chemical Communications, 47 (6), 1839-1841.

Abstract
3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to 90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.
Citation Information
Dannon J. Stigers, Zachary I. Watts, James E. Hennessy, Hye-Kyung Kim, et al.. "Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis" (2011)
Available at: http://works.bepress.com/nick_dixon/60/