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Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies
Faculty of Science - Papers (Archive)
  • Peter S.C. Wu, Australian National University
  • Kiyoshi Ozawa, University of Wollongong
  • Siew P. Lim, Novartis Institute for Tropical Diseases Pte Ltd
  • Subhash G. Vasudevan, Novartis Institute for Tropical Diseases Pte Ltd
  • Nicholas E Dixon, University of Wollongong
  • Gottfried Otting, Australian National University
RIS ID
16854
Publication Date
1-1-2007
Publication Details

Wu, P. S.C., Ozawa, K., Lim, S. P., Vasudevan, S. G., Dixon, N. E. and Otting, G. (2007). Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies. Angewandte Chemie (International Edition), 46 (18), 3356-3358.

Abstract
Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h.
Citation Information
Peter S.C. Wu, Kiyoshi Ozawa, Siew P. Lim, Subhash G. Vasudevan, et al.. "Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies" (2007) p. 3356 - 3358
Available at: http://works.bepress.com/nick_dixon/52/