Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studiesFaculty of Science - Papers (Archive)
AbstractQuick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h.
Citation InformationPeter S.C. Wu, Kiyoshi Ozawa, Siew P. Lim, Subhash G. Vasudevan, et al.. "Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies" (2007) p. 3356 - 3358
Available at: http://works.bepress.com/nick_dixon/52/