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A Single subunit directs the assembly of the escherichia coli DNA sliding clamp loader
Faculty of Science - Papers (Archive)
  • Ah-Young Park, University of Oxford
  • Slobodan Jergic, University of Wollongong
  • Argyris Politis, University of Oxford
  • Brandon T. Ruotolo, University of Cambridge
  • Daniel Hirshberg, University of Cambridge
  • Linda L. Jessop, University of Wollongong
  • Jennifer L. Beck, University of Wollongong
  • Daniel Barsky, University of Cambridge
  • Mike O'Donnell, Rockefeller University
  • Nicholas E Dixon, University of Wollongong
  • Carol V. Robinson, Universiry of Oxford
RIS ID
32229
Publication Date
1-1-2010
Publication Details

Beck, J. L., Robinson, C. V., Park, A. Young., Barsky, D., Hirshberg, D., Ruotolo, B. T., Politis, A., O'Donnell, M., Jergic, S., Dixon, N. E. & Jessop, L. L. (2010). A Single subunit directs the assembly of the escherichia coli DNA sliding clamp loader. Structure, 18 (3), 285-292.

Abstract

Multi-protein clamp loader complexes are required to load sliding clamps onto DNA. In Escherichia coli the clamp loader contains three DnaX (τ/γ) proteins, δ, and δ′, which together form an asymmetric pentameric ring that also interacts with ψχ. Here we used mass spectrometry to examine the assembly and dynamics of the clamp loader complex. We find that γ exists exclusively as a stable homotetramer, while τ is in a monomer-dimer-trimer-tetramer equilibrium. δ′ plays a direct role in the assembly as a τ/γ oligomer breaker, thereby facilitating incorporation of lower oligomers. With δ′, both δ and ψχ stabilize the trimeric form of DnaX, thus completing the assembly. When τ and γ are present simultaneously, mimicking the situation in vivo, subunit exchange between τ and γ tetramers occurs rapidly to form heterocomplexes but is retarded when δδ′ is present. The implications for intracellular assembly of the DNA polymerase III holoenzyme are discussed.

Grant Number
ARC/LX0882706
Citation Information
Ah-Young Park, Slobodan Jergic, Argyris Politis, Brandon T. Ruotolo, et al.. "A Single subunit directs the assembly of the escherichia coli DNA sliding clamp loader" (2010)
Available at: http://works.bepress.com/nick_dixon/47/