Cell-free synthesis of 15N-labeled proteins for NMR studiesFaculty of Science, Medicine and Health - Papers
AbstractModern cell-free in vitro protein synthesis systems present powerful tools for the synthesis of isotope-labeled proteins in high yields. The production of selectively N-15-labeled proteins from N-15-labeled amino acids is particularly economic and yields are often sufficient to analyze the proteins very quickly by two-dimensional NMR spectra recorded of the crude reaction mixture without concentration or chromatographic purification of the protein. We review methodological aspects of cell-free in vitro protein synthesis based on an Escherichia coli cell extract, in particular with regard to the production of N-15-labeled proteins for analysis by NMR spectroscopy.
Citation InformationKiyoshi Ozawa, Nicholas E Dixon and Gottfried Otting. "Cell-free synthesis of 15N-labeled proteins for NMR studies" (2005)
Available at: http://works.bepress.com/nick_dixon/44/