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Selected Works of Nicholas Snow
Article
Extraction of Glucocorticoids From Water Into Acetonitrile Using Polyol Induced Extraction with Ultra Performance Liquid Chromatography and Triple Quadrupole Mass Spectrometry (PIE-UPLC-MS-MS)
Journal of Liquid Chromatography and Related Technologies (2021)
  • Shipra Patel, Seton Hall University
  • Nicholas Snow, Seton Hall University
Link
Abstract
Polyol-induced Extraction (PIE) was used for the extraction and UPLC-MS-MS was used for the analysis of glucocorticoids in water. The focus of this work was to demonstrate PIE as an effective technique that allows extraction of solutes between miscible solutes using a phase separating agent, with an example analysis of glucocorticoids. In this work glycerol is used as the mass separating agent in the extraction of glucocorticoids from water into acetonitrile. Eight glucocorticoids were extracted by PIE and analyzed by UPLC-MS-MS. Extraction conditions were optimized, and extraction results were compared to non-extracted glucocorticoid standards and to literature methods. Extraction was optimized at 0 °C, in 10 mL of a 1:1 ACN/Water mixture 2 mL of glycerol added as phase separating agent. Calibration curves showed overall correlation of r2 > 0.99. Precent relative standard deviation below 4%was obtained for all analytes. Limits of detection were obtained between 3–4 ng/mL by the IUPAC method and 40–200 ng/mL by a propagation of errors method. PIE is demonstrated to be a simple and quick method for extracting glucocorticoids from water into a miscible polar solvent, acetonitrile.
Keywords
  • Glucocorticoids,
  • liquid chromatography,
  • PIE,
  • polyol induced extraction,
  • UPLC-MS-MS
Disciplines
Publication Date
2021
DOI
10.1080/10826076.2022.2110116
Publisher Statement
Citation Information
Shipra Patel and Nicholas Snow. "Extraction of Glucocorticoids From Water Into Acetonitrile Using Polyol Induced Extraction with Ultra Performance Liquid Chromatography and Triple Quadrupole Mass Spectrometry (PIE-UPLC-MS-MS)" Journal of Liquid Chromatography and Related Technologies Vol. 44 Iss. 17-20 (2021) p. 831 - 841 ISSN: 1520-572X
Available at: http://works.bepress.com/nicholas_snow/76/