Article
Determination of Total Polyvinylpyrrolidone (PVP) in Ophthalmic Solutions by Size Exclusion Chromatography with Ultraviolet-visible Detection
Journal of Chromatographic Science
(2011)
Abstract
A straightforward size exclusion chromatography (SEC) method was developed and validated for the determination of total polivinylpyrrolidone (PVP) in ophthalmic solutions using the unusual combination of size exclusion chromatography (SEC), ultraviolet-visible detection and quantitation of an analyte peak that elutes in the total exclusion volume of the column. Samples of opthalmic solutions are diluted with water and injected onto a TSKgel G1000PW, 7.5 mm i.d. × 30 cm, 12 µm column at 50°C, with 80:20 0.1M sodium acetate-methanol mobile phase and UV detection at 220 nm. Validation was successful for a stability indicating pharmaceutical method, with parameters including specificity, accuracy, linearity, and precision within typical pharmaceutical acceptance criteria. A stress study with acid, base, peroxide, heat, and light indicates that there is no interference from drug, product, or excipients.
Disciplines
Publication Date
July, 2011
DOI
10.1093/chrsci/49.6.457
Citation Information
Nicholas Snow, Panagiotis Tavlarakis and John J. Urban. "Determination of Total Polyvinylpyrrolidone (PVP) in Ophthalmic Solutions by Size Exclusion Chromatography with Ultraviolet-visible Detection" Journal of Chromatographic Science Vol. 49 Iss. 6 (2011) p. 457 - 462 Available at: http://works.bepress.com/nicholas_snow/51/