Analysis of Steroids using Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry-Mass Spectrometry (SPME-GC-MS-MS)Scientia Chromatographica (2014)
Direct immersion SPME-GC-MS-MS was used for the analysis of steroids in water at part-per-trillion(ppt) and lower concentrations. The method was validated and extended to real sample analysis. The method were linear from 0.01 to 5 ng/ml with precision less than 10% relative standard deviation for a steroid mixture at 1 ng/ml. Limit of quantitation and limit of detection was found to be 200- 1200 pg/L and 30-200 pg/L respectively and recoveries ranged from 88-103 %. To understand the extraction efficiency of the fiber, a depletion study was performed. The fiber/ sample partition coefficients for the steroids were determined to be 1.0 x 104 to 1.5 x 104. The extraction was performed without derivatization or the use of an internal standard. SPME-GC-MS-MS effectively demonstrated ultra-trace level detection of steroids in water.
- water analysis,
Citation InformationShilpi Chopra, Paulo CF Lima Gomes, Ramkumar Dhandapani and Nicholas Snow. "Analysis of Steroids using Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry-Mass Spectrometry (SPME-GC-MS-MS)" Scientia Chromatographica Vol. 6 Iss. 2 (2014) p. 105 - 116
Available at: http://works.bepress.com/nicholas_snow/38/